Device

Part:BBa_K4375016:Design

Designed by: Zska Csorba   Group: iGEM22_ELTE   (2022-09-29)


anti-EGFR Nanobody Display System


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 29
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2029
    Illegal BamHI site found at 4045
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2050
    Illegal NgoMIV site found at 4066
    Illegal AgeI site found at 4124
    Illegal AgeI site found at 4256
    Illegal AgeI site found at 4640
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We codon optimised for production in E.coli


Source

[1] Reprogramming Synthetic Cells for Targeted Cancer Therapy Boon Lim, Yutong Yin, Hua Ye, Zhanfeng Cui, Antonis Papachristodoulou, and Wei E. Huang ACS Synthetic Biology 2022 11 (3), 1349-1360 DOI: 10.1021/acssynbio.1c00631

References